Chapter 11 Lab Exercises

Section 3 Building a Biofilm Model from a Confocal Microscope Stack

Page 3 Student

Copyright © Alfred B. Cunningham, John E. Lennox, and Rockford J. Ross, Eds. 2001-2010

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Building a Biofilm Model from a Confocal Microscope Stack

Student Version (go to Instructor Version)

Build a Model

The ten images, Frame 1 through Frame 10, in the next section represent different layers in a biofilm, from the base of the biofilm to the top, respectively. These images were constructed from a confocal microscope, a special tool that can capture images at different levels in a biofilm. Your instructor will guide you in the creation of a styrofoam image reconstruction of the original biofilm.

A Confocal Image Stack of a Biofilm

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 1

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 2

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 3

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 4

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 5

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 6

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 7

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 8

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 9

Biofilm Lifecycle

Permissions

Permission pending, P. Stoodley, Center for Biofilm Engineering, Montana State University, Bozeman

Frame 10

Biofilms: The Hypertextbook

1 Biofilms: The Hypertextbook

Contents

Chapters

1 Introduction to Biofilms

2 Biofilm Growth and Development

3 Beneficial and Naturally Occurring Biofilms

4 Biofilms in Health and Medicine

5 Biofilms in Industrial Environments

6 Characterizing and Understanding Biofilms

7 Controlling Biofilms

8 Methods for Studying Biofilms

9 Mathematical Models of Biofilms

10 Diffusion in Biofilms

11 Lab Exercises

Sections

1 Overview and Instructions

2 Building and Using a Batch Biofilm Growth Reactor (Mason Jar Reactor)

3 Building a Biofilm Model from a Confocal Microscope Stack

4 Building and Using a Biofilm Continuous Flow Stirred Reactor

5 Building and Using a Flow Cell Biofilm Reactor

6 Collecting Soil Biofilms by the Buried Slide Technique

7 Colorimetric Measurement of Biofilm Density

8 Drop Plate Method for Counting Biofilm Cells

9 Flow Through Gram Stain

10 Harvesting and Dispersing of Cells from Biofilms (Standard Method)

11 Harvesting and Dispersing of Cells from Biofilms (Alternative Method)

12 Henrici's Microbial Fishing Technique

13 Measurement of Biofilm Thickness

14 Model System for Growing a Standard Biofilm: Static Glass Coupon Reactor

15 Biofilms as Biobarriers

16 Dental Biofilms: Detection and Quantification of Plaque

17 Isolation of Biofilm Populations from Soil Crumbs by Flotation

18 Bacterial Infection in a Cystic Fibrosis Patient

19 Buccal Epithelial Cells with Adherent Bacterial Cells Revealed by Negative Staining

20 Construction of a Winogradsky Column

21 Constructing and Using a Drip Flow Reactor

22 Coaggregation: Interactions between Bacteria in Dental Biofilms

23 Antimicrobial Sensitivity: Biofilm vs. Planktonic Cells

24 Detection of Naturally Occurring Acyl- Homoserine Lactones Using a Chromobacterium Reporter Strain

Pages

Appendices

1 Aids for Instructors

Help

1 Read Me First

2 System Requirements