Bacterial Infection in a Cystic Fibrosis Patient

1 Biofilms: The Hypertextbook

1 Contents

1 Introduction to Biofilms

2 Biofilm Growth and Development

3 Beneficial and Naturally Occurring Biofilms

4 Biofilms in Health and Medicine

5 Biofilms in Industrial Environments

6 Characterizing and Understanding Biofilms

7 Controlling Biofilms

8 Methods for Studying Biofilms

9 Mathematical Models of Biofilms

10 Diffusion in Biofilms

11 Lab Exercises

       
1 Overview and Instructions

       
2 Building and Using a Batch Biofilm Growth Reactor (Mason Jar Reactor)

       
3 Building a Biofilm Model from a Confocal Microscope Stack

       
4 Building and Using a Biofilm Continuous Flow Stirred Reactor

       
5 Building and Using a Flow Cell Biofilm Reactor

       
6 Collecting Soil Biofilms by the Buried Slide Technique

       
7 Colorimetric Measurement of Biofilm Density

       
8 Drop Plate Method for Counting Biofilm Cells

       
9 Flow Through Gram Stain

       
10 Harvesting and Dispersing of Cells from Biofilms (Standard Method)

       
11 Harvesting and Dispersing of Cells from Biofilms (Alternative Method)

       
12 Henrici's Microbial Fishing Technique

       
13 Measurement of Biofilm Thickness

       
14 Model System for Growing a Standard Biofilm: Static Glass Coupon Reactor

       
15 Biofilms as Biobarriers

       
16 Dental Biofilms: Detection and Quantification of Plaque

       
17 Isolation of Biofilm Populations from Soil Crumbs by Flotation

       
18 Bacterial Infection in a Cystic Fibrosis Patient

       
19 Buccal Epithelial Cells with Adherent Bacterial Cells Revealed by Negative Staining

       
20 Construction of a Winogradsky Column

       
21 Constructing and Using a Drip Flow Reactor

       
22 Coaggregation: Interactions between Bacteria in Dental Biofilms

       
23 Antimicrobial Sensitivity: Biofilm vs. Planktonic Cells

       
24 Detection of Naturally Occurring Acyl- Homoserine Lactones Using a Chromobacterium Reporter Strain

1 Instructor

2 Student

1 Aids for Instructors

2 References

1 Read Me First

2 System Requirements

3 Navigating